射频消融大鼠肝癌对树突状细胞的影响

Influence of radiofrequency ablation on dendritic cells in rats with liver carcinoma

目的分析大鼠肝肿瘤射频消融（RFA）后树突状细胞（DC）的变化，探讨RFA诱发机体抗肿瘤免疫应答的机制。方法40只健康SD大鼠建立肝癌模型，随机分为对照组（1（1只）、治疗7d组（16只）和治疗14d组（14只）。对照组不做治疗直接处死取材，后两组分别在RFA治疗后7d和14d处死取材。取外周血、肿瘤周边或消融灶周边0．5cm内肝组织及脾组织，采用流式细胞术检测树突状细胞表面OX62、OX6、CD86表达水平。结果①对照组OX62阳性细胞占外周血单个核细胞比率为（0．45±0．19）%，治疗7d组和14d组分别增高为（0．78±0．30）％、（1．53±0．80）％，与对照组相比差异均有统计学意义（P〈0．05）。②对照组瘤周肝组织内OX62阳性细胞比率为（18．91±4．58）％，治疗7d组升高为（24．49±4．45）％（P〈0．05）；对照组瘤周肝组织内DC表面OX6阳性表达率为（15．29±4．59）oA，治疗7d组和14d组分别增高为（34．20±11．62）％、（39．18±9．14）％，与对照组相比差异均有统计学意义（P〈0．05）。③对照组脾组织OX62阳性细胞比率为（11．69±4．39）％，治疗14d组增高至（15．10±2．37）％（P〈0．05）。结论RFA治疗肝癌后大鼠外周血DC前体细胞数量明显增多，肝局部和脾DC数量增多，OX6阳性表达率增高，表明射频治疗促进DC的分化成熟，提高机体抗原提呈功能，有利于宿主抗肿瘤免疫应答的恢复。

Objective To investigate the change of dendritic cells （DCs） in rats with hepatic carcinoma treated by radiofrequency ablation （RFA）,and to explore the mechanisms of anti tumor immune response to RFA. Methods Forty healthy SD rats with established animal model of hepatic carcinoma were randomly divided into control group （ n = 10）,RFA 7 d group （ n = 16） and RFA 14 d group （ n = 14）. The rats of control group were killed without treatment. The other rats were killed in 7 d and 14 d after RFA treatment respectively. Peripheral blood, liver tissue around the ablation area and spleen were taken out. The OX62,OX6,CD86 of DCs were analyzed with flowcytometry. Results ①OX62 cells accounted for （0. 45± 0.19） % of mononuclear cells in peripheral blood in control group. The account of OX62 cells increased to （0.78± 0.30）% and （1.53 ± 0.80）% in RFA 7 d and 14 d groups respectively. There were significant differences between control and RFA 7 d group, control and RFA 14 d group （ P d0.05）. ②OX62 cells accounted for （18.91 ±4.58）% of mononuclear cells in liver tissue around the tumor in control group. The account of OX62 cells increased to （24.49 ± 4. 59） % in RFA 7 d group （ P〈0.05）. The expression of OX6 onDCs in liver tissue was （15. 29 ± 4. 59） % and increased to （34.2± 11.62）% and （39.18±9. 14）% in RFA 7 d and RFA 14 d group respectively （ P 〈0.05）. ③OX62 cells accounted for （11.69 ±4.39）% of mononuclear cells in spleen in control group which increased to （15.10 ± 2.37）% in RFA 14 d group （P 〈0.05）. Conclusions The precursor DCs in peripheral blood and DCs in liver and spleen increased significantly after RFA. The expressions of OX6 on DCs in liver and spleen increased after RFA. RFA can promote the differentiation and maturation of DC. The increased function of antigen presenting may contribute to the anti tumor responses after RFA.