再生障碍性贫血患者免疫抑制性受体PD-1的作用

Role of programmed death-1(PD-1) in patients with aplastic anemia

目的检测程序性死亡1(PD-1)和可溶性PD-1(sPD-1)在再生障碍性贫血(AA)患者的表达,探讨其在AA发病机制中的作用。方法采用已建立的鼠抗人PD-1分子单克隆抗体,用流式细胞术检测PD-1在AA患者(AA组)及正常人(对照组)T淋巴细胞表面的含量;用人sPD-1酶标检测sPD-1在AA患者及正常人外周血血清中的含量。结果AA组外周血T淋巴细胞上无PD-1表达,而sPD-1水平与对照组相比明显增高(P<0.01)。结论AA患者的PD-1从活化T淋巴细胞膜上脱落导致sPD-1高表达,使PD-1缺如而无法发挥负性调控作用,导致T细胞活化功能持续亢进,引起骨髓造血功能衰竭。

Objective To study the levels of programmed death-1 （PD-1） and soluble PD-1 （sPD-1） in peripheral blood of patients with aplastic anemia （AA） and explore their role in developing AA. Methods The expressions of PD-1 and sPD-1 were detected by flow cytometry in patients and healthy volunteers as the controls. Results The expression of sPD-1 in AA patients was significantly higher than that in the controls （P〈0.05）, but AA patients had no expression of PD-1. Conclusion PD-1 falls off from the activated lymphocyte membrane, which leads to sPD-1 overexpression and absent expression of PD-1 in AA patients. Constent activation of T cells may cause dysfunction of marrow hemopoiesis.