HSP60、HSP70、HSP90α在结直肠癌组织中的表达及其与病理组织学分级的关系

Expressions of heat shock protein(HSP) family HSP 60,70 and 90α in colorectal cancer tissues and their correlations to pathohistological characteristics

背景与目的:结直肠癌是常见的恶性肿瘤之一,其发病的分子机制有待进一步阐明。目前,HSPs家族成员在某些恶性肿瘤的发生发展中的作用已有一些报道,但是这一家族成员HSP60、HSP70、HSP90α在结直肠癌组织中的表达还未见报道。本课题旨在研究结直肠癌组织中HSP60、HSP70、HSP90α的mRNA及其蛋白的表达,并分析其与病理分级的关系。方法:采用RT-PCR、免疫组化及Westernblot方法测定49例结直肠癌及其癌旁组织中HSP60、HSP70、HSP90α的表达;分析其与结直肠癌的病理组织学类型及分化程度的关系。结果:结直肠癌组织中HSP60、HSP70、HSP90αmRNA及蛋白的表达均显著高于相应的癌旁组织。癌组织较其癌旁组织HSP60、HSP70和HSP90α表达率及过表达率均有明显升高。在蛋白水平证明三种HSPs在癌组织中的表达明显高于癌旁组织。但是,HSP60、HSP70及HSP90α在结直肠癌高分化腺癌、中分化腺癌、低分化腺癌的表达率和过表达率差异均无统计学意义。Western-blot表明HSP60、HSP70、HSP90α在结直肠癌组织较癌旁组织中的表达也明显增高。结论:结直肠癌组织HSP60、HSP70、HSP90α三种基因的mRNA及蛋白表达均明显高于癌旁组织,但是其与病理分级无相关关系。

Background and Objective. Colorectal cancer is the third common malignant tumor in the world. Heat shock protein （HSP） family has been reported to play an important role in carcinogenesis of various cancers.However, little is known about expressions of HSP60,HSP70 and HSP90α in colorectal cancer. This study was to investigate expressions of HSP 60, 70 and 90cc, and analyzed their correlations to pathohistologic characteristics in colorectal cancer. Methods. Colorectal cancer tissues and adjacent normal tissues 2 cm away from the tumor focus were collected from 49 patients. Expressions of HSP60, HSP70 and HSP90α mRNA were detected by RT-PCR. The protein expressions of HSP60, HSP70 and HSP90α were determined by immunohistochemistry and western blot. Results. The mRNA and protein levels of HSP60, HSP70 and HSP90α, as well as their positive rates were significantly increased in tumor tissues compared with those in para-cancerous tissues. The overexpression rates of HSP60, HSP70 and HSP90α were also significantly higher in the colorectal cancer tissues than those in the corresponding para-cancerous tissues. The positive and overexpression rates of HSP60, HSP70 and HSP90α in well, moderately and poorly differentiated colorectal cancer were not significantly different. Conclusions. HSP60, HSP70 and HSP90α may play important roles in the pathogenesis of colorectal cancer, although they are not correlated with the pathological grading.