高效液相色谱-质谱联用技术在中药鉴定中的应用

HPLC-MS Technology in the Application of the Traditional Chinese Medicine Appraisal

中药鉴定学是一门传统而古老的学问,是随着中医理论的建立和发展,中药鉴定学已成为一门系统而完善的学科。近年,国家制定了中药现代化发展规划。为了保证中药的质量,各种现代的鉴定法越来越多地在中药鉴定中被应用。利用高效液相色谱-质谱(HPLC-MS)联用技术,将HPLC对复杂样品的高分离能力与MS的高选择性、高灵敏度以及能够提供分子量与结构信息的优点结合起来,可对中药质量进行比较全面的评价。本文就HPLC-MS联用技术近年来在中药材和中药制剂指纹图谱研究中的应用作一综述。

The traditional Chinese medicine appraisal study is a tradition, but the ancient knowledge, is along with the Chinese medicine theory establishment and the development, the traditional Chinese medicine appraisal study has become the discipline which a system consummates. Recent years, the country has worked out the traditional Chinese medicine modernization development proiect. In order to guarantee that the traditional Chinese medicine the quality, each kind of modem appraisal law more and more is applied in the traditional Chinese medicine appraisal. Uses highly effective liquid chromatography - mass spectrum （HPLC-MS） to unite with the technology, HPLC to the complex sample＇s high separating power and the MS high selectivity, the high sensitivity as well as can provide the molecular weight and the structure information merit unifies, may carry on quite comprehensive appraisal to the traditional Chinese medicine quality. This article united on HPLC-MS with the technology in recent years should serve as a summary in the traditional Chinese medicine and in the traditional Chinese medicine preparation fingerprint atlas research.