再生障碍性贫血患者外周血白细胞介素8水平的检测

Detection of interleukin8 level in peripheral blood of patients with aplastic anemia

目的：探讨白细胞介素８（ＩＬ－８）在再生障碍性贫血（再障）发病机制中的作用及与临床状况间的关系。方法：采用夹心酶联免疫吸附法检测２４例再障患者及２０名正常人外周血上清中ＩＬ－８水平。结果：再障患者ＩＬ－８水平显著高于正常对照（Ｐ＜０．０５），且重型再障组较慢性再障组显著增高（Ｐ＜０．０５），合并感染的再障患者ＩＬ－８含量升高更为显著（Ｐ＜０．００５）。结论：作为细胞趋化因子及造血负性调控因子的ＩＬ－８，在再障的病理过程中可能起着一定的作用，并对判断病情的严重程度及感染的存在具有一定的价值。

Objective: To investigate the role of interleukin8 in the pathogenesis of aplastic anemia(AA) and its correlation with clinical status.Methods:Serum level of IL8 in 24 AA patients and 20 normal controls was measured by sandwich enzyme linked immunosorbent assay(ELISA).Results:The levels of IL8 were increased significantly(P<0.05) in AA patients than in normal controls, and the IL8 levels in severe AA group were much higher than those in chronic AA group (P<0.05). Serum IL8 in AA patients with infection were elevated significantly(P<0.005). Conclusion: IL8 may have a potential role in the pathogenesis of AA and determination of serum IL8 may be helpful for evaluation of severity and infectious complication of AA.