G6PD缺乏红细胞膜磷脂含量及其不对称性改变的研究

Changes in the contents of membrane phospholipid and membrane phospholipid asymmetry in Glucose6phosphate dehydrogenase deficient erythrocyte

目的：通过测定葡萄糖－６－磷酸脱氢酶（Ｇ６ＰＤ）缺乏红细胞膜磷脂的含量及其不对称性的变化，研究Ｇ６ＰＤ缺乏对红细胞膜结构的影响。方法：不对称性实验采用磷脂酶Ａ２处理完整红细胞，磷脂的测定采用高效液相色谱法。结果：正常红细胞膜磷脂酰乙醇胺（ＰＥ）、磷脂酰丝氨酸（ＰＳ）、磷脂酰胆碱（ＰＣ）含量分别为５．５９±１．１６，２．９２±０．５０，６．６４±０．９２ｍｇ／ｍｇ膜蛋白；Ｇ６ＰＤ缺乏红细胞膜ＰＥ、ＰＳ、ＰＣ分别为５．１８±１．８６，１．５１±０．５８，５．７０±１．９１ｍｇ／ｍｇ膜蛋白，两组中ＰＳ有显著性差异（Ｐ＜０．００１），说明Ｇ６ＰＤ缺乏红细胞膜ＰＳ含量降低。在正常人红细胞膜中有１７．６％±２．５％ＰＥ，５７．９％±４．７％ＰＣ在外层，外层未发现ＰＳ，在Ｇ６ＰＤ缺乏红细胞膜中有３０．２％±５．７％ＰＥ，５３．８％±７．４％ＰＣ，２７．２％±５．８％ＰＳ在外层，两组中ＰＥ和ＰＳ有显著性差异（Ｐ＜０．０１和Ｐ＜０．００１），外层的ＰＣ无明显变化，说明ＰＥ、ＰＳ均向外层移动。结论：由于Ｇ６ＰＤ缺乏，红细胞膜磷脂结构发生改变，是红细胞溶血的重要机制之一。

Objective:To study the effect of G6PD deficiency on membrane phospholipid structures. Methods: Treatment of intact erythrocyte with phospholipase A2 was used for the membrane phospholipid asymmetry. Membrane phospholipid was determined by high performance liquid chromatography (HPLC). Results: The contents of phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS) were 5.18±1.86, 1.51±0.58 and 5.70±1.91mg/mg membrane protein, respectively, in G6PD deficient erythrocytes and were 5.59±1.16,2.92±0.50 and 6.64±0.92mg/mg membrane protein,respectively, in normal erythrocytes. In normal erythrocytes, 17.6%±2.5% PE and 579%±4.7% PC were present and no PS was found on the outer leaflet of the membrane. While in G6PD deficient ones, 30.2%±5.7% PE, 53.8%±7.4% PC and 27.2%±5.8% PS were present on the outer leaflet. Conclusion: Membrane phospholipid changes in G6PD deficient erythrocytes might be one of the factors causing hemolysis. 