雷公藤甲素对人子宫内膜癌抑制作用的裸鼠体内研究

Growth inhibition effect of triptolide on human endometrial cancer cells in vivo

目的:探讨雷公藤甲素(triptolide)对人子宫内膜癌细胞株HEC-1B裸鼠皮下移植瘤的生长抑制作用及其作用机制。方法:将人子宫内膜癌细胞株HEC-1B细胞接种于裸鼠皮下,建立HEC-1B皮下移植瘤模型,然后随机分为雷公藤甲素高中低剂量组8、4、2μg/(只·天)和顺铂40μg/(只·天)及生理盐水对照组共5组,治疗10～15天,观察治疗前后裸鼠体重及移植瘤体积的变化。SABC免疫组织化学染色法检测移植瘤组织中Bcl-2及内皮细胞生长因子(vascular endothelial growth factor,VEGF)的表达。结果:高剂量雷公藤甲素组和顺铂用药组抑制肿瘤作用强,抑瘤率都为50%;高剂量雷公藤甲素组中的Bcl-2及VEGF的表达明显低于生理盐水对照组(P<0.05)。结论:雷公藤甲素对裸鼠皮下移植瘤有明显的生长抑制作用,其机制可能与其诱导肿瘤细胞凋亡及抑制肿瘤血管形成有关。

Objective：To investigate effece of triptolide on the growth inbibitio of human endometrial cancer and to find out the role of triptolide on the expressions of Bcl-2 and VEGF. Methods：Human endometrial cancer cell line HEC-1B was seeded in the subcutaneous and a implant xenograft model was established by using nude mice. Mice were randomly divided into five groups,then treated with 0.9% NaCl solution,high,middle,and low dose triptolide （8,4,2 μg/d each one） and DDP 40μg/d each one for up to 10-15 days respectively. Tumor size was measured in 2 dimensions at regular time-intervals and the expressions of Bcl-2 and VEGF in tumor tissues were detected by SABC. Results：After treatment, the size of the xenografts in high-dose triptolide group were 894.802＋ 81.519 mm^3, compared with1771.702＋114 mm^3 in the control group （P 〈 0.05,With inhibition rate of 50%）. The expressions of Bcl-2 and VEGF in this group were significantly lower than that in the control group （P 〈 0.05）. Conclusion：High dose triptolide can significantly inhibit the growth of the xenografts of endometrial cancer HEC-1B in nude mice. The mechanism may be closely related to down-regulating the expressions of Bcl-2 and VEGF in tumor cells by triptolide.