福莫特罗和ICI118551对大鼠骨髓间充质干细胞来源的成骨样细胞功能的影响

The effects of Formoterol and ICI118551 on rat osteoblast-like cells in vitro induced from marrow-derived mesenchymal stem cells

目的:研究选择性β2肾上腺素能激动剂福莫特罗(Formoterol)和阻滞剂ICI118551对体外由大鼠骨髓间充质干细胞(MMSC)成骨分化的影响,探讨β2肾上腺素能受体信号对骨代谢的影响。方法:取3周龄雌性SD大鼠,全贴壁筛选法培养MMSC,用条件培养液诱导向成骨细胞分化后分别加入不同浓度Formoterol和ICI118551,检测细胞碱性磷酸酶,骨钙素的分泌水平和细胞增殖率。结果:MMSC成功诱导分化为成骨样细胞,不同浓度(10-5～10-9mmol/L)的Formoterol均可抑制成骨样细胞分泌碱性磷酸酶,骨钙素和细胞增殖。高浓度ICI118551(10-5和10-6mmol/L)抑制成骨样细胞分泌碱性磷酸酶,骨钙素和细胞增殖,低浓度ICI118551(10-8和10-9mmol/L)则可促进成骨样细胞分泌碱性磷酸酶,骨钙素和细胞增殖。结论:β2肾上腺素能受体激动剂可抑制体外成骨样细胞的功能和细胞增殖,而阻滞剂对成骨样细胞功能和增殖的影响与其浓度有关。

Objectlve：To evaluate the effects of Formoterol（β2-adrenergic receptor-specific agonist） and IC1118551（β2-adrenergic receptor-specific antagonist） on rat osteoblast-like cells induced from marrow-derived mesenchymal stem cells （MMSC）. Methods： MMSCs by whole bone marrow adherent culture derived from a three-week-old female SD rat are induced to osteoblast-like cells. After different concentrations of Formoterol and IC1118551 are added into the culture medium,thealkali phosphatase and osteocalcin is detected. Results：The alkali phosphatase,osteocalcin and proliferation of cells cultured with all different concentrations（10^-5-10^-9 mmol/L） of Formoterol are obviously low. As to IC1118551, high concentrations （10^-5 and 10^-6 tool/L） can inhibit the expression of the alkali phosphatase,osteocalcin and proliferation of cells while low concentrations （10^-8 and 10^-9 mol/L） can enhance the expression of the alkali phosphatase, osteocalcin and proliferation. Conclusion：The β2-adrenergic receptor-specific agonist can inhibit the activity of rat osteoblast-like cells in vitro while the effects of the β2-adrenergic receptor-specific antagonist are not single, depending on its concentration.