碱性成纤维细胞生长因子对肺癌A459细胞pim-3表达影响的实验研究

Effect of basic fibroblast growth factor on pim-3 in humanl lung cancer cell A459

目的:探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,FGF-2)对肺癌A459细胞株pim-3的影响。方法:不同浓度的(0、25、50、100ng/ml)FGF-2在体外作用于肺癌A459细胞,半定量逆转录聚合酶链式反应(RT-PCR)法检测pim-3mRNA,Western blot检测pim-3蛋白表达。结果:与空白对照组比较,RT-PCR和Western blot检测结果显示pim-3mRNA及其蛋白的表达在各FGF-2作用组均显著增高,各组间比较差异有统计学意义(P<0.01)。结论:FGF-2可以量效的方式上调pim-3的表达。

Objective：To investigate the effect of basic fibroblast growth factor（FGF-2） on pim-3 in humanl lung cancer cell A459. Methods：Lung cancer cell A459 was treated by different concentrations（0、25、50、100 ng/mL） of FGF-2,pim-3 mRNA was measured by reverse transcription polymerase chain reaction（RT-PCR）,and western blotting was used to detect the expression of PIM-3 protein. Results：The expressions of pim-3 mRNA and protein increased dramaticly in the groups treated with FGF-2 compared with conorol,and there was a significantly difference between each two experiment groups（P 〈 0.01）. Conclusion：FGF-2 can significantly upregulate the expression of pim-3 in dosage-dependent manner.