吡柔比星和顺铂联合应用对人卵巢癌3AO细胞系的作用

Effect of Pirarubicin Combined with Cisplatin on Human Ovian Cancer 3AO Cell Line

目的:观察吡柔比星和顺铂联合应用对人卵巢癌3AO细胞系的抑制效应,并初步探讨其作用机制。方法:用MTT (四甲基偶氮唑盐)法检测化疗药物对人卵巢癌3AO细胞系的抑制率(Fa);FCM(流氏细胞术)检测肿瘤细胞周期分布和细胞凋亡。结果:吡柔比星和顺铂单独用药可时间和浓度依赖性抑制3AO细胞的生长。IC_(50)(半数抑制浓度)随时间延长而明显减小。联合应用吡柔比星和顺铂后抑制率呈时间和浓度依赖性。两药的IC_(50)也比单独用药时显著降低;吡柔比星和顺铂合用呈协同作用;两药单用和联合应用对细胞周期均有影响,细胞凋亡率均提高。结论:两药联合呈协同作用,药物的作用均与其诱导凋亡和影响细胞周期有关,但对细胞周期的影响各不相同。

Objective： To study the combination effect and antitumor mechanism of pirarubiein and cisplatin on human ovian cancer 3AO cell line in vitro. Method： MTT assay was used to analyze the effect of chemotherapy drugs on the growth of human ovian cancer 3AO cell line. Flow cytometry （FCM） was used to observe the cell cycle and apoptosis. Result： Pirarubicin and cisplatin alone or in combination inhibited the growth of human ovian cancer 3AO cell line in a time- and concentration-dependent fashion. IC50 were decreased. The combination index was less than 1 at 24h, 48h, and 72h, synergistic effect of pirarubicin and cisplatin in growth inhibition of human ovian cancer 3AO cell line was indicated. The pirarubicin and cisplatin had effects on the cell cycle and apoptosis. Conclusion： Pirarubicin and cisplatin has synergistic effect on human ovian cancer 3AO cell line. All the drugs can change the distribution of cell cycle and induce apoptosis.