HPLC法测定脑得生软胶囊中葛根素和羟基红花黄色素A的含量

Determination of Puerarin and Hydroxysafflor Yellow A in Naodesheng soft Capsules by HPLC

目的:建立脑得生软胶囊中葛根素和羟基红花黄色素A的含量测定HPLC方法。方法:色谱柱Hypersil C_(18)(250 mm×4.6 mm,5μm),流动相甲醇-乙腈-0.2%磷酸溶液(16:3:81),检测波长250 nm(测葛根素)、403 nm(测羟基红花黄色素A),流速1.2 ml·min^(-1)。结果:葛根素和羟基红花黄色素A的线性范围分别为32～162μg·ml^(-1)(r=0.999 9)和7.3～116.8μg·ml^(-1)(r=0.999 9),其回收率分别为101.3%(RSD=1.1%)和99.8%(RSD=1.3%,n=6)。结论:方法简便、灵敏、重复性好,可作为脑得生软胶囊的质量控制方法。

Objective： To develop a RP-HPLC methods for the determination of puerarin and hydroxysafflor yellow A in Naodesheng soft capsules. Method： The Hypersil C18 column （250 mm × 4.6 mm ,5μm） was used ,the methanol- acetonitrile-0.2% phosphoric acid solution （ 16：3： 81 ） as mobile phase, the flow rate both were 1.2 ml·min^ - 1 for determination of puerarin, the detection wavelength was 250 nm. and for determination of hydroxysafflor yellow A. the detection wavelength was 403 nm. Result： The linear ranges of puer- arin and hydroxysafflor yellow A were 32 - 162 μg·ml ^-1 （ r = 0.999 9 ） and 7.3 - 116.8 μg·ml ^- 1 （ r = 0.999 9）, the average recoveries （n = 6） were 101.3% RSD 1.1% ,and 99.8% RSD 1.3%, respectively. Conclusion： The methods is simple, sensitive and repeat-able, it can be used for qualiity control of Naodesheng soft capsules.