摘要
目的研究胆囊胆固醇结石病人肝脏的肝脏受体类似物1（Liver receptor homolog 1，LRH-1）及其调控的三磷酸腺苷结合盒（ABC）转运体家族成员abcg5／8的表达，探讨胆固醇结石病发病的机制。方法27例胆囊胆固醇结石病人，男6例，女21例；年龄平均52岁。10例无胆石症的胆囊息肉病人为对照，男6例，女4例；平均年龄48岁。测定胆汁脂类成分和计算胆汁胆固醇饱和指数。实时定量PCR法测定肝脏LRH-1及abcg5／8mRNA的表达量。结果胆石组LRH-1表达高于对照组（14．18±1．80VS7．22±2．22，P〈0．05），胆石组肝脏abcg基因mRNA表达量均较对照组增高（abcg5：49．34±3．68vs33．48±2．77，P〈0．05；abcg8：38．93±5．70vs18．70±3．42，P〈0．05）。胆石组胆汁呈胆固醇过饱和（1．17±0．02）。结论该研究结果提示人类肝脏LRH-1及其调控的abcg5／8的表达增高与胆囊胆固醇结石形成有关。
Objective To investigate the mRNA expression of liver receptor homolog 1 （LRH- 1） gene and abcg5/8 genes regulated by LRH-1 in patients with cholesterol gallstone disease to elucidate the biomolecular pathogenesis of gallstone formation. Methods Twenty-seven patients with cholesterol gallstone （CGS） and 10 controls without gallstones were enrolled in this study. The biliary composition was assayed, mRNA expression of LRH-1 and abcg5/8 genes was determined by RTPCR. Results The expression levels of LRH-1 and abcg5/8 genes were significantly higher in CGS patients than in controls （LRH-1 ： 14. 18±1.80 vs 7.86±2.22; abcg5 ：49. 34 ±3.68; abcg8 ：38. 93± 5.70 vs 18.70±.42; P〈0.05）. Conclusion CGS disease may be related to increased expression of LRH-1 and abcg5/8 genes.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2009年第5期325-328,共4页
Chinese Journal of Hepatobiliary Surgery
基金
国家自然科学基金资助项目（30271272）
