摘要
包涵体是指细菌表达的蛋白在细胞内凝集,形成无活性的固体颗粒。对于从事生化研究的科研工作者而言,包涵体的纯化是一个十分重要和棘手的问题,结合笔者的实践经验,简述了包涵体的纯化中需要注意的问题。
Inclusion is the expression of proteins in bacterial ceils agglutinate, the formation of the solid particles inactive. Engaged in research for chemical and biological research workers, the inclusion body purification is an important and difficuh issue, the article combined with practical experience of the author, outlines the purification of inclusion bodies in the need to pay attention to.
关键词
包涵体
纯化
处理
inclusion
purification
processing