摘要
通过实验确定了粗柄羊肚菌(Morchella crassipes)原生质体制备的最佳条件,即:采用培养72 h的菌丝,以0.6 mol/L NaCl作为稳渗剂配制的4 mg/L蜗牛酶,5 mg/L溶壁酶的混合酶液为细胞壁降解酶液,30℃,50 rpm/min酶解3 h,可得原生质体最高产量为2.07×106个/100 mg/mL。以0.6 mol/LNaCl溶液做稳渗剂,MYG再生培养基上得到原生质体再生率为0.16%。
The experiment to determine the rough handle morel （MoreheUa crassipes） the best protoplast preparanon eonomons, namely： the use of 72 h the myeelium culture to 0.6 tool / L NaGl as a solid preparation for dialysis dose 4 nag / L snail enzyme, 5 mg/ L of mixed soluble enzyme wall enzyme for cell wall degrading enzyme solution, 30 ℃, 50 rpm / min digestion 3 h, the highest available output of protoplast 2.07 × 106 months / 100 nag / mL. To 0.6 tool / L NaCl solution so steady infiltration agent, MYG regeneration medium on protoplast regeneration rate to be 0.16%.
关键词
粗柄羊肚菌
原生质体
酶解
原生质体产量
再生率
morehella handle rough
protoplasts
enzymolysis
protoplast yield
regeneration rate
