摘要
用包含GAI基因的EHA105农杆菌,对巴西橡胶树海垦2花药愈伤组织进行侵染,对多项转化条件进行了梯度实验,结果表明选择培养30 d的橡胶花药愈伤组织,农杆菌菌液浓度OD600为0.6时侵染3 min,20℃共培养2 d,Cef浓度为200 mg/L进行抑菌培养是比较合理的转化条件。
The Agrobacterium containing GAI gene was used to infect the anther callus of Haiken 2 - a variety of Hevea brasiliensis to get a transgenie plant. The gradient experiments of several transformation conditions were done. The results showed that： the anther callus cultured for 30 days, 0.6 OD600 value of the Agrobacterium solution, 3min infection time, co -culture for 2 days at 20℃ and 200 mg/L Cef concentration for Agrobacterium inhibition culture were optimal transformation conditions.
关键词
巴西橡胶
农杆菌
GAI基因
Hevea brasiliensis
Agrobacterium
GAI gene
