平菇浸出液及其成分对结核杆菌生长的影响

Effect of cap fungus extract on the growth of Mycobacterium tuberculosis

目的研究以平菇浸出液及其分离的脂溶性和水溶性溶液代替蒸馏水制作改良罗氏培养基,对结核杆菌生长的影响。方法分别以平菇浸出液及其分离的脂溶性和水溶性溶液代替蒸馏水制作改良罗氏培养基,罗氏培养基作为对照,培养卡介苗中的结核杆菌。结果结核杆菌在平菇液罗氏培养基的生长情况最好,其次是水溶性平菇液罗氏培养基,在罗氏培养基和脂溶性平菇液罗氏培养基上的生长情况相对较差,而后者更差一些。结论单独加入平菇分离液中的脂溶性萃取液或非脂溶性萃取液对结核杆菌的生长均有一定的促进作用,但并不明显,两者只有按适当的比例混合时才能发挥协同作用,促进结核杆菌的生长。

Objective To investigate the effect of cap fungus extract on the growth of Mycobacterium tuberculosis. Methods Cap fungus extract was used to make modified Lowenstein-Jensen culture medium for cuhivanting Mycobacterium tuberculosis in BCG vaccine. Results The growth of Mycobacterium tuberculosis on cap fungus extract Lowenstein-Jensen culture medium was the best, followed by water-solubility solution Lowenstein-Jensen culture medium, Lowenstein-Jensen culture medium and liposolubility solution. Conclusion The liposolubility and water-solubility solution added with cap fungus extract can promote the growth of Mycobacterium tuberculosis.