摘要
目的探讨国产紫杉醇体外对人卵巢癌HO8910细胞凋亡的作用。方法MTT染色法测定HO8910细胞的生长,用流式细胞仪观察凋亡及细胞周期的变化。结果紫杉醇与HO8910细胞抑制作用呈浓度依赖关系,IC50=16.66umol/L。人卵巢癌细胞在紫杉醇作用下,没有出现明显凋亡。细胞分裂阻滞在G2M期。结论紫杉醇能有效抑制HO8910细胞生长,但是没有引起细胞凋亡。
Objective To investigate the mechanism of taxol on human ovarian cancer cell in vitro. Methods The effect of taxol on HO8910 cell was observed by cytometry and the apoptosis and cell cycle of HO8910 cell were determined. .Results The effectⅠt of taxol on human ovarian cancer cells was in a dose-dependent manner. The IC50 was up to 16.66um. No apeptosis of HO8910 cell was observed after treated with taxol. The mitosis of HO8910 cell treated with taxol arrested at metaphase of mitosis. Conclusion Taxol can effectively inhibit the growth of HO8910 cell but not induce apeptosis.
出处
《中国热带医学》
CAS
2009年第6期1089-1090,共2页
China Tropical Medicine
