埃兹蛋白表达与肝细胞癌增殖及转移的关系研究

Relationship between the expression of Ezrin and proliferation and metastasis of hepatocellular carcinoma

目的研究人肝癌组织中埃兹蛋白(Ezrin)的表达情况,探讨其与黏附分子E-cadherin、增殖活性指标PCNA及临床特征的相关性。方法49例原发性肝细胞癌及其癌旁肝组织标本按肿瘤大小、有无播散灶及远处转移、有无包膜、有无门静脉癌栓等分为高侵袭和低侵袭2组,采用免疫组织化学方法检测Ezrin、E-cadherin与PCNA的表达情况,并与远离癌旁的正常肝组织进行比较,探讨其与肝癌临床特征的关系。结果Ezrin在肝癌组织、癌旁组织和正常肝组织中均有表达,其在肝癌组织中的表达显著高于癌旁组织和正常肝组织(P<0.01),在高侵袭组中的表达显著高于低侵袭组(P<0.05);E-cadherin在肝细胞癌组织中的表达低于癌旁组织和正常组织(P<0.01),而在癌旁组织和正常肝组织中的表达无明显差异;在肝癌组织中,Ezrin的表达与E-cadherin呈负相关(r=-0.507,P<0.01),与PCNA呈正相关(r=0.543,P<0.01)。肝癌组织中的PCNA表达显著高于癌旁组织和正常肝组织(P<0.01),高侵袭组肝癌组织中的PCNA表达显著高于低侵袭组肝癌组织(P<0.05),而癌旁组织和正常肝组织中的表达无明显差异。结论Ezrin在肝癌中呈高表达,与肝癌高侵袭性、高增殖活性相关,且与E-cadherin表达下降所致的低黏附共同在肝细胞癌的侵袭和转移过程中发挥重要作用。

Objective To explore the expression of Ezrin in hepatocellular carcinoma （HCC）, and the relation of Ezrin expression to E-cadherin, proliferating cell nuclear antigen （PCNA） and clinical features of HCC. Methods Forty-nine cases of HCC were divided into high and low invasive groups according to tumor size, disseminated lesions, distant metastasis, tumor capsule and portal vein tumor thrombus. The expression of Ezrin, E-cadherin and PCNA in normal liver tissues, HCC and adjacent tissues were determined by immunohistochemistry, and the relation of the expression to clinical features was analyzed. Results The Ezrin expression was found in normal liver tissues, HCC and adjacent tissues, and was significantly higher in HCC tissues than in adjacent and normal liver tissues （P〈0. 01）, and was higher in high invasive group than in low invasive group （P〈0. 05）. The expression of E-cadherin in HCC tissues was significantly lower than in adjacent and normal liver tissues （P〈0. 01）, while no significant difference was found between adjacent and normal liver tissues. The expression of Ezrin was negatively correlated to that of E-eadherin （r= -0. 507, P〈0. 01）, and positively correlated to that of PCNA （r=0. 543, P〈0. 01）. The expression of PCNA was significantly higher in HCC tissues than in adjacent and normal liver tissues （P〈0. 01）, and was higher in high invasive group than in low invasive group （P〈0. 05）, while no significant difference was found between adjacent and normal liver tissues. Conclusion Ezrin is highly expressed in HCC tissues and correlated to the invasive ability and proliferative activity of HCC. Ezrin, together with the low adhesion induced by down-regulated E-cadherin expression, may play an important role in invasion and metastasis of HCC.