地塞米松凝胶对大鼠经皮渗透性及其对缺血随意皮瓣成活的影响

The Percutaneous Penetration of Dexamethasone Gel on Rats and Its Effect on Survival of Ischemic Random Skin Flaps

目的:研究地塞米松(DXM)凝胶对大鼠的经皮渗透性及缺血随意皮瓣成活的影响。方法:制备0.1%、0.3%、0.5%DXM凝胶,分别在24h内测定DXM经大鼠离体皮肤的累积渗透量;将30只大鼠分为0.1%、0.3%DXM凝胶和空白凝胶对照组,各组缺血随意皮瓣外给予相应药物7d后统计皮瓣成活面积。结果:DXM的累积渗透量与DXM浓度和渗透时间呈正相关,3种浓度的DXM凝胶在皮肤组织的累积量无明显差异(P>0.05);与对照组比较,0.1%、0.3%DXM凝胶分别增加皮瓣成活面积16.4%、58.47%(P<0.05)。结论:DXM可经皮进入皮肤组织,其0.3%凝胶制剂可显著提高缺血随意皮瓣的成活面积。

OBJECTIVE： To study the percutaneous penetration of dexamethasone gel through rats and its effects on survival of ischemic random skin flaps. METHODS： 0.1%, 0.3% and 0.5% dexamethasone gel were made. The accumulative penetration quantities of dexamethasone across rat skin in 24 h were assayed in vitro. 30 rats were random divided 3 groups including 0.1% ,0.3% dexamethasone gel and blank gel （control group） to use drugs by skin flaps for 7d ,then survival areas of flaps were measured by planimetry. RESULTS： The accumulative penetration quantities of dexamethasone increased in time- and concentration- dependent manner. No obvious difference in the accumulative quantities in skin of 3 groups （P 〉 0.05） . Compared with control group, the survival area was improved in 0.1% and 0.3% gel by 16.4% and 58.47% （P〈0.05） . CONCLUSIONS： Dexamethasone in gels might penetrate into skin tissues, 0.3% dexamethasone gel could significantly increase survival area of ischemic random skin flaps.