HPLC测定环香酮滴眼液中秦皮甲素的含量

Determination of aesculin in Huanxiangtong eye drops by HPLC

目的：建立环香酮滴眼液中秦皮甲素含量测定方法。方法：HPLC采用YMC C18柱（250mm×4．6mm，10μm），乙腈-0．1％磷酸（12：88）为流动相，流速为1．0mL·min^-检测波长为334nm，柱温为30℃。结果：秦皮甲素在0．0284～0．284μg范围内线性关系良好（r=0．9996），平均加样回收率为99．16％，RSD为0．89％；测得每瓶含秦皮甲素大干1．51mg。结论：该方法重现性好，准确度高，可作为环香酮滴眼液定量分析方法。

Objective： To establish an HPLC method for determining aesculin in Huanxiangtong eye drops. Methods： The samples were analyzed by HPLC using a YMC Cls column （250 mm ×4.6 mm,10 μm） , the mobile phase was consisted of acetonitrile-0.1% phosphoric acid （ 12：88 ） at the flow rate of 1.0 mL. min^-1 , the detection wave-length was at 334 nm, and the column temperature was 30 ℃. Results： A linear range of aesculin curve was within 0. 028 4 -0. 284 0μg（r =0. 999 6） , the average recovery was 99. 16% and the RSD was 0.89%. The content of aesculin was 1.51 mg per bottle. Conclusion： The method is simple, reproducible and accurate, therefore it can be used for the quality control of Huanxiangtong eye drops.