RP-HPLC法测定竹柏叶中穗花双黄酮含量

Determination of amentoflavone in Podocarpus nagi leves by RP-HPLC

目的：建立竹柏叶中德花双黄酮含量测定方法。方法：采用高效液相色谱法，色谱条件为：AgilentC18色谱柱（150mm×4．6mm，5μm）；乙腈-0．05％磷酸水液为流动相，梯度洗脱（时间0～30min，乙腈15％～100％）；流速为1．0mL．min^-1，检测波长为340nm。结果：穗花双黄酮在0．013—0．130μg范围内线性关系良好（r=0．9999），平均加样回收率为96．34％，RSD为1．4％（n=6）。结论：本法操作简便，结果准确，重现性好，可用于竹柏叶中穗花双黄酮成分的含量测定，并为其开发应用提供基础。

Objective ： To establish a method to determine the content of amentoflavone in Podocarpus nagi leves. Methods： An HPLC method was carried out with an Agilent Hypersil ODS column （150 mm ×4.6 mm, 5μm）. The mobile phases were acetonitrile （A） and 0.05% phosphoric acid solution （B） , and the gradient elution was performed in 0 -30 min with acetonitriie fiom 15% to 100%. The flow rate was 1.0 mL.min^-1 and the detection wavelength set at 340 nm. Results ： The linear range of amentoflavone was within 0.013 - 0.130 μg. The average recovery rate was 96.34% with RSD of 1. 4% （n = 6）. Conclusion： The method was simple, rapid, accurate and suitable for the detection of amentoflavone in Podocarpus nagi leves. The results provided a basis for the development and application of Podocarpus nagi leves.