外排泵抑制剂小肽1号对抗菌药抗菌活性的影响

The Effects of Efflux Pump Inhibitor No.1 Small Peptide on Antibacterial Activity of Antiseptic Drug

为了观察外排泵抑制剂小肽1号对抗菌药抗菌活性的影响,本试验采用标准微量稀释法,测定了6类8种抗菌药单用和与小肽1号联用对12株临床分离鸡大肠杆菌的MIC值。结果表明,12株鸡大肠杆菌中有9株为产超广谱酶的多重耐药菌株,小肽1号(1∶2)使恩诺沙星等8种药物的抗菌活性多数增强2倍,使氟苯尼考对A8、A15的抗菌活性增强了4倍,小肽1号(1∶2或1∶4)使恩诺沙星、甲替沙星、环丙沙星的抗菌活性增强2倍,使左旋氧氟沙星对A13的抗菌活性增强4倍。以上结果表明,细菌外排泵抑制剂-小肽1号对大多数药物的抗菌活性有一定的增强作用,产酶多重耐药的鸡大肠杆菌至少同时存在产ESBLs、外排泵两种耐药机制。

To observe the effects of efflux pump inhibitor-No. 1 small peptide on antibacterial activity of antiseptic drug, the minimal inhibitory concentrations of eight kinds of antiseptic drug treated with No. 1 small peptide alone and in combination to twelve strains E. coli isolated from chickens were measured with standard microdilution method. The results showed that nine of twelve chickens E. coli were multi-drug resistant isolates, which can produce extended-spectrum β-lactometers. With the effect of No. 1 small peptide （one to two）, the antibacterial activity of eight kinds of antiseptic drug, such as enrofloxacin, could be improved by twice and the antibacterial activity of Florfenicol against A8 and A15 was improved by fourth. With the effect of No. I small peptide （one to two or one to four）, the antibacterial activity of Enrofloxacin, Gatifloxacin and Ciprofloxacin could be improved by twice and the antibacterial activity of Levofloxacin against A13 was improved by fourth. These results showed that bacterial efflux pump inhibitors-No. 1 small peptide one could improve the antibacterial activity of most anti septic drugs and chickens 15.. coli which were produced enzyme and multi-drug resistanted has at least two resistance mechanisms of ESBLs and efflux pumps at the same time.