安卡鸡Mx基因A2032G变异位点对经济性状的效应分析

The Genetic Effects of A2032G Mutation Point of Mx Gene on Some Commercial Traits in Anka Chicken

采用错配PCR-RFLP对安卡鸡Mx基因2032位点多态性进行检测,结果表明,安卡鸡Mx基因2032位点经RsaⅠ酶切后,检测到AA、AG和GG 3种基因型,基因型频率分别为0.232、0.681和0.087,抗性等位基因A的频率为0.572。χ2适合性检验结果表明,安卡鸡Mx基因在RsaⅠ酶切位点上等位基因的分布显著偏离Hardy-Weinberg平衡状态。分析这3种基因型与安卡鸡一些经济性状间的关联性,结果表明,抗性纯合子AA基因型的个体各阶段体重、体尺、主要屠宰性状及常规肉质与AG和GG基因型的个体差异不显著,安卡鸡Mx基因2032位点对重要经济性状没有显著的负效应。

The Mx gene in Anka chicken was analyzed with mismatch PCR RFLP method, the results showed that M2032 locus of Mx gene digested by restriction endonuclease Rsa Ⅰ could be divided into three kinds of genotypes, AA, GG and AG. The frequencies of these genotypes were 0. 232, 0. 681 and 0. 087, respectively. The frequency of resistance allele A was 0. 572. The result of Chi Square test indicated that the frequencies of Mx gene digested by Rsa I were significantly deviated from Hardy-Weinberg equilibrium in this study. The correlation between the three genotypes with some commercial traits was analyzed, the results showed that the body weight of different developmental stage, body size mainly slaughter traits and meat quality of the individuals were with resistant genotype AA were not significant different with those individuals with AG and GG genotypes in Anka chicken, which indicated that M2032 locus of Mx gene had no significantly negative genetic effects on commercial traits in Anka chicken.