淡色库蚊三氟氯氰菊酯抗性株的选育及交互抗性的研究

SELECTION OF RESISTANT STRAIN OF CULEX PIPIENS PALLENS TO LAMBDA CYHALOTHRIN AND ITS CROSS RESISTANCE TO OTHER INSECTICIDES

本研究在实验室条件下进行淡色库蚊（Ｃｕｌｅｘｐｉｎｐｉｅｎｓｐａｌｅｎｓ）三氟氯氰菊酯抗性品系的选育，观察抗性的发展趋势，测定交互抗性谱，分析抗性品系的种群生存力、吸血率、孵化率等主要生物学特征，并比较了两个品系的相对适合度。结果发现：淡色库蚊对三氟氯氰菊酯的抗性经６代的选育后达到４１．８倍。前４代稳步上升，第５代（Ｆ４）有所下降，以后又继续攀升，抗性的发展出现波动：通过对抗性品系（Ｒ）和敏感品系（Ｓ）生长、发育、生殖等观察发现：Ｒ品系在孵化率、化蛹率、羽化率和产卵率等方面均明显低于Ｓ品系，对有关参数进行统计分析发现Ｒ品系相对Ｓ品系仅具０．４１的适合度；经６代选育所得的三氟氯氰菊酯抗性品系对三种拟除虫菊酯药物有明显交互抗性，尤其是与二氯苯醚菊酯交互抗性达４０倍以上，与溴氰菊酯、氯氰菊酯的交互抗性也在２０倍以上，与两种有机磷类杀虫剂马拉硫磷、敌百虫无交互抗性，与氨基甲酸酯类的残杀威不仅没有交互抗性，还稍有下降。这些为进一步研究蚊虫对拟除虫菊酯的抗性机理打下了基础，为防治提供了一些依据。

This paper deals with the results of selecting resistant strain of Culex pipiens pallens to lambda cyhalothrin and its cross resistance to other insecticides.It was found that the resistance to lambda cyhalothrin increased quickly,the LC50 reached to 13.3pb from 0.32ppb(41.76 fold) after 6 generations.The bloodfeeding,oviposition and surviving rates declined in larvae and pupae period.Its fitness value was just 0.41 related to the susceptible strain(s).Further more,this lambda cyhalothrin resistant strain showed high cross resistance to some pyrethroids,including permethrin(41 folds) ,deltamethrin(22 folds) and cypermethrin(28 folds),but no cross resistance was found to trichlorfon,malathrion and propoxur.