保幼激素Ⅲ对三带喙库蚊滞育发生期间卵巢滤泡和体内RNA变化的影响

Effect of Juvenile Hormone Ⅲ on Ovarian Follicle Development andthe Content of RNA of Culex tritaeniorhynchus Giles in Diapause

目的：探索保幼激素Ⅲ（ＪＨ３）对滞育三带喙库蚊卵巢滤泡和体内ＲＮＡ变化的影响。方法：短光照和低温联合作用导致三带喙库蚊滞育后，用２ｐｐｍ０．５ｕｌ或３ｐｐｍ０．５ｕｌ／蚊外源ＪＨ３点滴处理，观察卵巢滤泡发育情况，并测定蚊体内ＲＮＡ和卵巢内蛋白质含量的变化。结果：低温和短光照引起三带喙库蚊滞育后，卵巢滤泡发育受到抑制，体内ＲＮＡ的含量明显减少，ＪＨ３处理可诱导部分滞育的三带喙库蚊转入发育状态，解除滞育的个体卵巢第一滤泡增大，体内ＲＮＡ含量增加，但对卵巢蛋白的含量没有影响。结论：ＪＨ３可诱导滞育三带喙库蚊卵巢发育，体内ＲＮＡ合成增加。

Ovarian follicle development was retarded and content of RNA decreased after diapause of Culex tritaeniorhynchus Giles occured.The present paper is the results of effect of Juvenile hormone Ⅲ on ovarian follicle development and the content of RNA of Culex tritaeniorhynchus Giles in diapause under experimental condition.It was showed that the smaller follicles in ovaries enlarged and the content of RNA increased after topical treatment with synthetic Juvenile hormone Ⅲ,and it was not found the influence of Juvenile hormone Ⅲ on content of protein in ovarian.