美洲拟鲽抗冻蛋白基因转化番茄的形态学与苹果酸脱氢酶同工酶检测

The morphology and isoenzymes analysis of inansgenic tomato introduced antifreeze protein gene

将带有afp基因的Ti质粒pMon(232)，作为供体DNA，用花柱道和子房注射DNA的方法转化番茄品种“中蔬四号”对D1D2代进行田间抗寒性观察，发现在春季平均气温低于正常年分4．4℃条件下，有些组分田间长势明显好于对照。初步确认已获得抗寒性。对D2代转基因植株进行MDH同工酶分析，均与对照有明显差异，间接验证了外源DNA的导入，说明同工酶分析，可作为转基因植株早期筛选的生化指标。

Antifreeze protein gene (afp), which is integrated into the Ti plasmid pMon, ^(232)was introduced into tomato (Zhong Shu 4) via the pollen - tube. pathway and ovary injection. The assay of cold resistance in the field showed that the transgenic plants of D_1 and D_2 generation could grow better than control obviously under the average temperature that is 4.4℃ below normal year in earlier spring, and which affirmed that the transgenic tomato plants have gained cold resistance. The analysis of MDH isoenzymes of D_2 generation showed that obvious differences between transgenic plants and contrul plants, which indirect proved that foreign gene has been introduced into tomato, meanwhile indicated that the analysis of isoenzymes can be used as one of the methods for earlyscreening of transgenic plant.