神经元凋亡的离体模型及其检测技术

In Vitro Models of Neuronal Apoptosis and the Detective Methods.

近年来，随着细胞凋亡研究的深入，神经元凋亡与神经退变病的关系愈发引人注目，已建立多种神经元凋亡的离体模型．多种因素如营养剥夺、自由基、谷氨酸、低钙及β淀粉样蛋白等均可诱发神经元凋亡．凋亡的检测，可先从酶或蛋白质的变化判断神经元的损伤情况，再结合形态学观察。

Recently, attention has been focused on the association between neuronal apoptosis and neurodegenerative diseases during the deepgoing study of apoptotic cells. Many in vitro models of neuronal apoptosis have been developed, such as induced by nutrition deprivation, free radicals, glutamic acid, Ca^2+ ,β-amyloid peptide etc. The detective technology of neuronal apoptosis is composed of testing the changes in enzymes and proteins which reflected neuronal damage, simultaneous morphological observation, and finally DNA electrophoresis etc, which could confirm the apoptosis.