光照、湿度和培养基对苎麻疫霉卵孢子产生量的影响

Effects of illumination, moisture and medium on oospore production of Phytophthora boehmeriae .

采用３因素随机区组设计研究了光照、湿度和培养基对苎麻疫霉（Ｐｈｙｔｏｐｈｔｈｏｒａｂｏｅｈｍｅｒｉａｅ）卵孢子产生量的影响．结果表明，各因子对卵孢子产生量的影响效应大小次序为培养基＞光照＞湿度，其中培养基和光照两因子影响均在０．０１水平上显著．在供试的４种常用培养基上，卵孢子产生量的大小次序为：ＳＬＡ培养基（ＳＬＡ）＞利马豆培养基（ＬＢＡ）＞Ｖ６汁培养基（Ｖ６Ａ）＞澄清的Ｖ６汁培养基（Ｖ６Ｂ）．在设置的３种光照条件中，卵孢子产生量以连续黑暗处理最高，连续光照最低，光照与黑暗交替处理居中．３个试验因子间的所有互作对卵孢子产生量均有极显著的影响．在不同光照、湿度、培养基组合中，卵孢子产生量以低湿＋连续黑暗＋ＳＬＡ组合最高，低湿＋连续光照＋Ｖ６Ｂ组合最低．

The effects of illumination, moisture and medium on the oospore production (OP) of Phytophthora boehmeriae were studied in vitro by three factors randomized block design. The results indicated that the order of the effects of the test factors was medium>illumination>moisture, and the effects of both medium and illumination were significant at 0.01 level.On the media tested, the number of formed oospore was decreased with the order of SLA medium (SLA)>lima bean agar medium (LBA)>V 6 juice medium (V 6A)>clarified V 6 juice medium (V 6B). Of the three illumination conditions designed, OP from the continuous darkness treatment was the highest, next was 12h illumination +12h darkness, and the lowest was from the continuous illumination treatment.The interactive effects of the three factors on OP were all significant at 0.01 level. Among different combinations of illumination, moisture and medium, OP from low moisture+continuous darkness+SLA was the highest, and that from low moisture +continuous illumination+V 6B was the lowest.