芍药苷对血管紧张素转化酶活性的抑制作用

Inhibitory Effects of Paeoniflorin on ACE Activity In Vitro

目的:采用反相高效液相色谱法(RP-HPLC)测定了芍药苷对血管紧张素转化酶活性的影响。方法:采用Brava BDS C18色谱柱(4.6mm×250mm,5μm),流动相为乙腈-0.01%甲酸水溶液(25:75),柱温为30℃,流速为1.0mL·min-1,检测坡长为228nm。结果:马尿酸在1.17～149μg·mL-1(r=0.9999)范围内呈良好的线性关系;平均回收率(n=6)为100.4%(RSD=0.88%);日内、日间精密度的RSD分别为1.32%和1.96%。芍药苷的IC50值为37.47μmol·L-1。结论:该法分离效果和重复性均良好,结果准确可靠,可用于马尿酸的检测,也为血管紧张素转化酶抑制剂活性测定提供了可靠的方法。

This is a study attempting to develop an efficient RP-HPLC method for determining the inhibitory activity of angiotensin converting enzyme （ACE）. The content of enzymolysis product hippuric acid was determined using RP - HPLC method. RP-HPLC was performed on a Brava BDS C18 column （4.6mm×250mm,5μm） using a mobile phase consisting of 0.01% formic acid solution-acetonitrile （25：75） at 30℃ with a flow rate of 1.0 mL·min^-1 and a detection wavelength at 228 nm. Test results showed that the calibration curve was hnear in the range of 1.17-149μg·mL^-1（r=0.9999） for hippuric acid. The average recovery （n=6） of hippuric acid was 100.4% （RSD=0.88%）. The intra-day and inter-day RSDs were at 1.32% and 1.96% respectively. The IC50 of Paeoniflorin was 37.47μg·mL^-1. Enjoying a sensitive, accurate, and reliable performance with good reproducibility, the method is desirable for determining the inhibitory activity of ACE.