2种泡桐的丛枝病器官体外植株再生系统的建立

Establishment of in vitro plantlet regeneration system by witches' broom organ of two Paulownia species

以毛泡桐及白花泡桐丛枝病叶片、叶柄和茎段为外植体,建立了其体外植株再生系统.结果表明,毛泡桐丛枝病叶片、叶柄和幼嫩茎段在MS+NAA1.1 mg.L-1+6-BA8 mg.L-1,MS+NAA0.1 mg.L-1+6-BA 4mg.L-1和MS+NAA0.5 mg.L-1+6-BA 12 mg.L-1培养基上,最高芽诱导率分别为73.88%,42.11%和25.00%;而白花泡桐的丛枝病不同器官的最适培养基分别为MS+NAA 0.3 mg.L-1+6-BA 12 mg.L-1,MS+NAA0.9 mg.L-1+6-BA16 mg.L-1和MS+NAA0.7 mg.L-1+6-BA20 mg.L-1,最高芽诱导率分别为91.11%,32.78%和13.33%.此外,1/2MS+NAA0.5 mg.L-1和1/2MS+NAA0.3 mg.L-1可分别作为毛泡桐和白花泡桐丛枝病幼芽根诱导的最适培养基.

Plantlet regeneration system was established with witches＇ broom leaves, petioles and nodal segments of Paulownia tomentosa and Paulownia fortunei. The results indicated that the highest shoot induction rates of the witches＇ broom leaves, petioles and nodal segments of P. tomentosa were 73.88%, 42.11% and 25.00% on the MS＋ NAA 1.1 mg·L^-1 ＋ 6-BA 8 mg·L^-1, MS＋NAA 0.1 mg·L^-1＋ 6-BA4 mg·L^-1 and MS＋ NAA0.5 mg·L^-1 ＋ 6-BA 12 mg·L^-1 meida respectively ; Those of P.fortunei witches＇ broom tissue were 91.11% , 32.78% and 13.33% on the MS ＋ NAA0.3 mg·L^-1 ＋ 6-BA 12 mg·L^-1,MS＋ NAA0.9 mg·L^-1＋ 6-BA 16 mg·L^-1 and MS＋ NAA 0.7 mg ·L^-1 ＋ 6-BA 20 mg ·L^-1 media respectively. Moreover,1/2 MS ＋ NAA 0.5 mg ·L^-1 and 1/2 MS ＋ NAA 0.3 mg·L^-1 were the optimal media for rooting.