摘要
从枣中分离得到粗蛋白聚糖,经DEAE-SepharoseCL-6B、SepharoseCL-6B和Sephadex G-200柱层析,分离纯化得白色粉末状枣蛋白聚糖(ZSG4b)。化学组成分析表明ZSG4b含83.5%多糖、9.7%蛋白质。ZSG4b在体外有明显的免疫活性,且活性随其浓度的增大而增加。
The crude water-soluble proteoglycan was isolated from Zizyphus jujuba cv. Jinsixiaozao.ZSG4b was obtained by purification using DEAE-SepharoseCL-6B anion exchange, Sepharose CL-6B and Sephadex G-200 column chromatography. ZSG4b contained 83.5% glycan and 9.7% protein. ZSG4b exhibited high immunity activity which was increased in accordance with the increase of concentration in vitro.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2009年第3期12-14,共3页
Food and Fermentation Industries
基金
江南大学食品学院青年博士科研创新开放基金(FS-200801)
食品科学与技术国家重点实验室资助
关键词
金丝小枣
蛋白聚糖
分离纯化
免疫活性
Zizyphus jujuba cv. Jinsixiaozao, proteoglycan, isolation and purification, immunity activity
