摘要
以小麦蛋白为原料,采用碱性蛋白酶和胰蛋白酶分步水解制备谷氨酰胺活性肽。通过单因素实验和正交实验得到的最佳制备条件为:碱性蛋白酶在温度60℃、pH9·5、底物浓度4%(w/v)、酶用量1·5%(w/w)的条件下水解2h,水解度为21·25%,再用胰蛋白酶在温度40℃、pH9·0、酶用量1·5%(w/w)的条件下水解2h,水解度提高3·30%。活性肽制品的得率为61·38%,肽中谷氨酰胺的含量为33·14%,三氯乙酸氮溶解指数为83·33%,HPLC法测定谷氨酰胺活性肽由相对分子量为2854和2117的两组分组成,峰面积百分比为35·69%和64·31%。
Glutamine-bioactive peptides were prepared by hydrolyzing wheat protein with Alcalase and Trypsin one by one.Through odd factor tests and orthogonal experiments,the optimum conditions of enzymatic hydrolysis were obtained as follows:Alcalase at 60℃,pH9.5,S% =4% (w/v) and E% = 1.5% (w/w) for 2h,DH =21.25% ; Trypsin at 40℃, pH9.0 and E% = 1.5% (w/w) for 2h, increasing DH = 3.30% .The final yield of bioactive peptides was 61.38%,the content of Gin was 33.14%, the TCA-NSI was 83.33%, the peptides was composed of relative molecular mass 2854 and 2117 by HPLC,the percentage was 35.69% and 64.31%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第5期184-187,共4页
Science and Technology of Food Industry
基金
安徽省科技攻关重大项目(07010301018)