明党参愈伤组织诱导及其细胞悬浮培养的研究

Research on callus induction and cell suspension culture of Changium smyrnioides

目的:建立明党参愈伤组织的诱导及其细胞悬浮培养的技术体系。方法:采用正交试验法研究不同植物激素对明党参叶柄、根和叶片愈伤组织诱导的影响,以及明党参不同外植体细胞的悬浮培养。结果:叶柄最易诱导出愈伤组织,其次是根和叶片,4种植物激素对明党参根和叶柄愈伤组织的诱导都具有极显著的效果,其中诱导叶柄愈伤组织形成的最佳激素组合为0.5 mg.L-1NAA+0.5 mg.L-12,4-D+0.1 mg.L-1KT+0.5 mg.L-16-BA,培养14 d左右,诱导率达到100%。此外,明党参叶柄细胞培养至21 d时,细胞量和多糖均达到最大值,分别1 090.28,36.85 mg.L-1。结论:明党参叶柄是愈伤组织诱导的最佳外植体和细胞悬浮培养体系的最佳细胞来源。

Objective： To establish the technical systems of callus induction and the cell suspension culture of Changium smyrnioides. Method：Effects of different plant growth regulators on leaf, petiole and root callus induction were studied by orthogonal design, and cell suspension cultures from different explants were carried out. Result：Petiole was optimal to form callus, followed by the root and leaf. Four kinds of plant growth regulators showed a significant effect on the petiole and root callus induction. The optimal plant growth regulators for petiole callus induction was 0.5 mg.L^-1 NAA,0.5 mg.L^-1 2,4-D,0.1 mg. L^-1 KT and 0.5 mg.L^-1 6- BA; And induction rate reached to 100% when petiole was cultured for 14 days. In addition, when cell from petiole callus was cultured for 21 days, the maximum yields of cell biomass and polysaccharide were 1 090.28 mg.L^-1 and 36.85 mg.L^ -1 , respectively. Conclusion ： Petiole was taken as the optimal explant for inducing callus and the optimal cell origin for the cell suspension culture of C. smyrnioides.