白芷酪氨酸酶抑制成分研究

Tyrosinase inhibitor from Angelica dahurica roots

目的:热处理白芷Angelica dahurica,并寻找其中的酪氨酸酶抑制成分。方法:采用生物活性-高效液相色谱跟踪方法,以及硅胶柱色谱法,分离、鉴定热处理白芷中酪氨酸酶抑制成分。结果:热处理白芷提取物比原药显示出更强的酪氨酸酶抑制作用,并从其有效部位中分离得到8-羟基-5-甲氧基补骨脂素(IC50=0.0086 mmol.L-1),比阳性对照曲酸(kojic acid)(IC50=0.0907 mmol.L-1)对酪氨酸酶抑制活性更强。结论:热处理明显提高了白芷酪氨酸酶抑制活性,8-羟基-5-甲氧基补骨脂素为活性成分。

Objective： To isolate and identify the tyrosinase inhibitory constituent from Angelica dahurica. Method： A tyrosinase inhibitory constituent was isolated by silica gel column chromatography combined with a bioassay-linked HPLC method. Result： An extract of heat-processed A. dahurica roots showed more potent tyrosinase inhibitory activity than that of the crude drug. Tyrosinase inhibitory furanocoumarin, 8-hydroxy-5-methoxypsoralen,was isolated. It express stronger tyrosinase inhibition than the known tyrosinase inhibitor, kojic acid （IC50 = 0. 090 7 mmol. L^- 1 ） with IC50 value of 0. 008 6 mmol.L^-1. Therefore,it is contributive to the increased tyrosinase inhibitory activity of the heat-processed A. dahurica. Conclusion： The active constituent was 8-hydroxy-5-methoxypsoralen from the heat-processed A. dahurica.