HPLC同时测定前胡中3种香豆素成分的含量

Simultaneous determination of three coumarin constituents in roots of Peucedanum praeruptorum by RP-HPLC

目的：建立了用HPLC法对前胡药材中白花前胡甲素、乙素和E素同时定量的方法。方法：高效液相色谱法,色谱柱Agilent TC-C18（4.6 mm×250 mm,5μm）;流动相甲醇-水（75∶25）;检测波长321 nm;柱温30℃;流速0.8 mL.min^-1;进样量20μL。结果：白花前胡甲素、乙素和E素的浓度与峰面积,分别在3.20-28.80μg（r=0.9999）,1.60~14.40μg（r=0.9995）,1.64~14.76μg（r=0.9994）呈良好的线性关系;平均加样回收率分别为98.92%,99.66%,99.72%,RSD分别为1.6%,1.5%,1.4%。结论：不同产地前胡所含的香豆素成分含量存在一定的差异,安徽宁国和祈门的前胡中白花前胡甲素含量高于药典的规定,且这3种香豆素含量明显高于其他2个产区。该法适合同时测定前胡样品中3种香豆素成分的含量,分离度好,快速,简便,重现性好。

Objective： To develop a reserved-phase HPLC method for the determination of praeruptorin A, praeruptorin B, qianhucoumarin E in roots of Peucedanum praeruptorum. Method： Agilent TC-C18 column （4. 6 mm×250 mm, 5 μm） was used at 30 ℃ with the mobile phase of methanol-water （75：25 ）. The flow rate was set at 0. 8 mL .min^-1. The detection wavelength was 321 nm. Result： The linear response ranged from 3.20-28. 80 μg for ± praeruptorin A （ r = 0. 999 9, n = 5）, 1.60-14. 40 g for praerup- torin B （r =0. 999 5, n =5） and 1.64-14. 76 g for qianhucoumarin E （r =0. 999 4, n =5）, respectively. Recoveries were 98. 92% with RSD 1.6% for praeruptorin A, 99. 66% with RSD 1.5% for praeruptorin B and 99.72% with RSD 1.4% for qianhueoumarin E. Conclusion ： The method is quick, simple and repeatable for determination of three coumarin constituents in root of P. praeruptorum.