金匮肾气丸、右归丸对肾阳虚小鼠模型以药反证的脑基因芯片研究

Gene chip study on cerebral gene of effect of Jinkui Shenqiwan and Youguiwan on mouse model of kidney-yang asthenia with syndrome disproved accroding to therapeutic efficacy of drugs used

目的:在基因水平上探讨补肾中药金匮肾气丸、右归丸对肾阳虚小鼠模型的作用机制。方法:昆明种小鼠分为正常组、模型组、金匮组和右归丸组。正常组和模型组每天灌胃蒸馏水0.5 mL;金匮组和右归丸组每天分别灌胃金匮肾气丸混悬液和右归丸混悬液0.5 mL,含生药129 mg.kg-1。造模组和治疗组均采用雄雌鼠比例1∶6同笼喂养,雄鼠每日游泳1次,直至无力下沉时捞出,达4周,以诱导产生"劳倦过度、房室不节"肾阳虚证。观察动物的畏寒、活动及反应、饮食和皮毛等情况。用36K mouse genome array鼠脑芯片检测正常组和肾阳虚模型组鼠脑基因,并以二者荧光信号相对强度比值≥2和≤0.5筛选差异显著基因,用qRT-PCR对部分差异表达基因进行验证。结果:模型组/正常组上调基因,二药治疗组/造模组基因下调23个基因,主要是与炎症/免疫、神经传递/信号转导等相关基因;造模组/对照组下调基因而治疗组/造模组基因上调的基因有6个,其主要是与细胞周期/细胞结构、神经传递/信号转导、转录等有关基因。qRT-PCR也证实了金匮组/模型组、模型组/正常组的GH和Scgb3a1的差异表达。结论:金匮肾气丸、右归丸可使肾阳虚小鼠模型显著下调的激素、黑色素显著上调并促进细胞增殖,从而在基因水平上探讨了金匮肾气丸和右归丸的药物作用机制及其差别。

Objective： To inquire into the cerebral gene change of effect of Jingui Shenqiwan and Youguiwan for animal model of kidney-yang asthenia caused by excessive physical and sexual activities, which may study the effect mechanism of the medicine with syndrome disproved therapeutic efficacy of drugs used. Method： Male mice of Kunming species, weight 35-40 g, and female weight 28- 35 g were randomly divided into four groups： control group, model group and treatment groups of Jigui Shenqiwan and Youguiwan in which there were ten male mice, fifteen ones, ten ones and ten ones, respectively. All of them were fed normally, and poured into their stomach with 0.5 mL of distill water for each mouse in the control group and model group, and with 0.5 mL suspension of the drugs（including 1.1 g.kg^-1 drug） for each one in the treatment groups every day. The mice in the model group and treatment groups were kept by means of each male mouse with six female mice in the same cage, and all male mice swam until they gradnlly submerged and were scooped up from water once everyday for lasting four weeks to induce the kidney-yang asthenia with excessive physical and sexual activities. Animals＂ manifestation such as fearing cold, activity and responses, mouse＂ fur and so on were observed. The brain gene were detected with the mouse brain gene chip of 36K Mouse genome array made by Capital Bio Corp. Beijing, China, and the differential expression gene were screened according to the ratio equal to or above 2 and equal to or below 0.5 with the related fluorescent intensity comparing the two groups, which could be further verified in the light of partly differential expression gene with qRT-PCR. Result： The mouse model of kidney-yang asthenia in the model group was successfully induced by way of excessive physical and sexual activities. There were twenty-three genes among up-regulated genes in the model group versus control group but down-regulated genes in the treatment groups versus model group, chiefly including the genes association with inflammation/immunization, neurotransmissions/ signal transduetion and so on. There were six genes among the down-regulated genes in the model group versus control group but upregulated in the treatment groups versus model group, mainly involving the related genes of cellular cycle and structure, neurotransmissions/signal transduction, transcription and et al. Conclusion： Jigui Shenqiwan and Youguiwan may make it markedly up-regulated to notably down-regulated genes of hormone and melanin-concentrating hormone（MCH） for model of mouse of kidney-yang asthenia, and promote cellular proliferation, which can inquire into effect mechanism of the drug in genetic level at the same time.