亚低温对高血压脑出血细胞凋亡的影响

The Effect of Mild Hypothermia for Apoptosis in Patient with Severe Hypertensive Intracerebral Hemorrhage

目的：观察高血压脑出血病人脑脊液中FasL的变化,研究亚低温对高血压脑出血病人神经细胞调亡的影响,探讨亚低温的脑保护机制。方法：根据入院时GCS评分选择重症高血压脑出血病人总共22例,被分随机为两组,亚低温治疗组,常规治疗组,采用酶联免疫吸附法1d、2d、3d、5d、7d,共5d测量脑出血病人脑脊液中可溶性FasL含量,用SPSS 13统计软件包分析比较两组结果。结果：在患者脑出血后1d内FasL含量开始升高,2～3d脑脊液中FasL含量达到高峰,并逐渐下降。亚低温治疗组患者脑脊液FasL含量明显低于常规治疗组（P〈0.05）。结论：亚低温能够减少高血压脑出血病人神经细胞调亡的发生,是其脑保护机制之一。

Objective ：To observe the change of the FasL in CSF of patient with hypertensive cerebral hemorrhage and to find mild hypothermia effects on apoptosis and to explore the mechanism of neuron protection. Method： A total of 22 patients with hypertensive cerebral hemorrhage were divided into 2 groups , mild hypothermia group （ MHTG , 10 cases） and conventional therapeutic group （ CTG, 12 cases ）. In MHTG , mild hypothermia was performed in 24 hours after intracerebral hemorrhage. We examined the levels of FasL in the cerebrospinal fluids （ CSF） of patients with cerebral hemorrhage using an enzyme - linked immunosorbent assay（ELISA）. Comparison analysis were performed by a SPSS 13 statistical package. Result： The concentrations of FasL were significantly elevated in 24hours and reached the highest level at about 48 - 72 hours after ICH, and gradually decrease. The concentrations of FasL in the MHTG group were lower than the CTG group. （ P 〈 0.05 ）. Conclusion： Mild hypothermia has the effect of protecting from nerve cell apoptosis.