乳杆菌调节小鼠空肠派伊尔结基因表达的通路分析

Analysis of modulation pathway on gene expression in mouse intestine Peyer's patches

目的:探索乳杆菌免疫作用机制。方法:BALB/c小鼠灌胃乳杆菌,从小鼠空肠派伊尔结提取RNA,基因芯片分析基因表达情况,基于已知的基因网络数据库利用Onto-Tools分析乳杆菌刺激引起的特异性基因网络。结果:Onto-Tools分析得到的通路中,涉及到免疫的通路有T细胞受体信号通路、B细胞受体信号通路、细胞因子和细胞因子受体通路、抗原加工和呈递等;涉及到粘膜屏障的有粘着斑、粘着连接、紧密连接和细胞粘附分子;还有涉及到细胞周期和凋亡的通路;其中通路中影响因子最高的为MAPK信号通路和粘着斑。结论:乳杆菌刺激引起大量蛋白表达,激活粘膜屏障,引起T细胞受体信号通路、B细胞受体信号通路、细胞因子和细胞因子受体通路等通路,从而造成免疫应答。

Objective： To investigate the mechanism that Lactobacillus modulates mouse immune responses. Methods： BALB/c mice were administrated with Lactobacillus for 14 days. Jejunal segment containing the Peyer＇s patch was isolated for gene expression analysis using genearray. Onto-Tools were used to explore significant pathway in database. Results： The analysis exhabited significant pathways involved in immune response： T cell receptor signaling pathway, B cell receptor signaling pathway, cytokine-cytokine receptor interaction and antigen processing and presentation etc. There were focal adhesion, adherisive junction, tight junction and cell adhesion molecules about mucosal barrier; Furthermore,there were cell cycle and apoptosis in pathways. The highest impact factors in analysis of pathway were MAPK signaling pathway and focal adhesion. Furthermore, there were cell cycle and apoptosis. Conclusion： Lactobacillus induces expression of considerable genes, which activates mucosal barrier, causing T cell receptor signaling pathway, B cell receptor signaling pathway, cytokine-cytokine receptor interaction etc and induces immune responses.