非淋菌性尿道炎的生殖支原体检测

Detection of Mycoplasma Genitalium in Nongonococcal Urethritis

目的 为了探讨生殖支原体 (Mycoplasmagenitalium ,Mg)感染与非淋菌性尿道炎 (NGU )的关系。方法 对 6 4例采自STD门诊的NGU患者尿道 (宫颈 )分泌物标本作了Mg检测。采用改良SP 4培养基作分离培养。结果与结论 实验结果表明 ,9例阳性 ,占检测总数 14 1%。其中男 6例 ,女3例。 2例合并解脲支原体感染 ,另 1例伴有沙眼衣原体感染。 9例阳性培养物经菌落形态观察、传代试验、生化反应、电镜观察及聚合酶链反应等鉴定试验证明 ,临床分离培养阳性株同Mg标准株 (G 3 7)的实验结果完全一致。

Objective To explore the relationship between Mycoplasma genitalium(Mg) infection and non gonococcal urethritis(NGU). Methods The discharge from urethra or cervical canal was tested in 64 patients with NGU at our STD clinic. Mycoplasma genitalium was isolated using SP 4 modified medium. Results and Conclusion The results indicated that Mg was found in 9 of 64 cases(14 1%) (6 men and 3 women). Two cases were coinfected with Ureaplasma urealyticum, and one with C. trachomatis. The Mg isolates were confirmed by colony morphology, subcuture test,biochemical reactions,electronmicroscopic observation and polymerase chain reaction. The isolates were consistent with Mg reference strain,G 37. The correlation between Mg and NGU were preliminarily discussed.