一例复发性生殖器疱疹的临床及其致病的HSV2野生株的糖蛋白D基因序列分析

Clinical Feature and Glycoprotein D Gene Sequence Analysis of a Wild Strain of HSV-2 Isolated from One Relapsed Patient with Genital Herpes

目的 探讨我国复发性生殖器疱疹的临床表现与糖蛋白D基因序列之间的关系。方法利用聚合酶链反应从我国一例复发性生殖器疱疹患者分离的野生株中扩增、克隆出糖蛋白D( gD)基因序列。结果 将野生株 gD基因序列同国外已发表的疱疹病毒 2G株进行比较 ,发现其gD基因的氨基酸序列有个别突变。结论 本课题对研究我国复发性生殖器疱疹的氨基酸序列和抗原结构 。

Objective To study the relation between the clinical feature and glycoprotein D gene sequence analysis of a wild strain of HSV 2 isolated from one relapsed patient with genital herpes. Methods The partial glycoprotein D gene sequence of the above mentioned strain was amplified and cloned with PCR. Results The comparison of the amino acid sequence of gD gene between the wild strain and HSV 2G strain published showed that there was a mutation at site80 and site159. Conclusion In order to develop effective vaccine which is suitable for China, it is necessary to investigate the differences of the gene structure and function of gD among HSV isolated from China and other countries.