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RNA干扰Tax基因表达及其对HTLV-Ⅰ在TLC中转录的影响 被引量:1

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摘要 目的探讨RNA干扰Tax基因表达对人嗜T淋巴细胞病毒Ⅰ型(HTLV-Ⅰ)在T淋巴细胞(TLC)中表达的影响。方法运用RNAi技术特异性干扰Tax基因的表达,流式细胞技术和western blot法鉴定Tax蛋白的表达情况,并通过p27特异性蛋白的检测反映HTLV-Ⅰ在TLC中表达。结果重组质粒psiTax/U6转染T细胞组Tax蛋白的表达(9.84±3.32)%较未处理组(47.45±14.52)%和pSilencer1.0/U6转染组(45.98±10.66)%明显降低;western blot法显示重组质粒psiTax/U6转染TLC后。Tax以及p27蛋白表达较pSilencer1.0/U6转染组明显降低。结论运用RNA技术干扰Tax基因表达可以有效的阻止HTLV-Ⅰ在TLC中的转录。
出处 《广东医学》 CAS CSCD 北大核心 2009年第6期885-887,共3页 Guangdong Medical Journal
基金 广东省医学科研基金资助项目(编号:B2007125)
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参考文献6

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同被引文献7

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