培养基成分和培养条件对黄芪愈伤组织中黄芪皂苷积累的影响

Effects of culture conditions on the saponin accumulation in Astragalus membranaceus callus

对提高黄芪愈伤组织中黄芪皂苷含量的人工调节技术进行了研究。生长在6,7-V基本培养基上的黄芪愈伤组织中黄芪皂苷含量比MS培养基上的高,而且光照比黑暗更有利于黄芪皂苷合成。调整6,7-V培养基中氨态氮和硝态碳的比例为1∶1(氮源总浓度为60 mmol.L-1)、KH2PO4浓度为1.5 mmol.L-1、CaCl2浓度为8.97 mmol.L-1时比较适合黄芪皂苷的积累。以蔗糖为碳源比葡萄糖和麦芽糖更有利于皂苷的积累。通过调节培养基成分和培养条件是改变黄芪细胞中黄芪皂苷积累趋势的一个重要手段。

The saponin accumulation in Astragalus membranaceus callus were studied. The saponin is higher when growing on 6,7-V than that growing on MS, and lumination was apt to stimulating saponin synthesis comparing with darkness. The better ratio of NH4^＋/NO3 for saponin accumulation in Astragalus membranaceus callus on 6,7-V medium is 1 ： 1 （total concentration of N was 60 mmol· L^-1）, in which KH2PO4 is 1.5 mmol · L^-1 and CaCl2 is 8.97 mmol· L^-1. Sucrose showed the stronger ability to induce saponin synthesis than glucose and maltose. The results suggested that the modulation of culture conditions is the better strategy to improve the saponin accumulation of Astragalus mernbranaceus callus.