^(99m)Tc标记双功能杂交单克隆抗体B72.3 MT-1的实验与临床研究

Preliminary clinical applications and experiments of 99m Tc labeled bifunctional hybrid molecule B723 MT1

应用基因工程方法将金属硫因（ＭＴ）表达到Ｂ７２．３单抗的Ｆｃ段上，制备出双功能杂交分子Ｂ７２．３ＭＴ－１。采用９９ｍＴｃ直接标记Ｂ７２．３ＭＴ－１显示标记过程简便、快速、标记率高，标记物稳定性好，不易脱落，适于药盒化生产。体外细胞结合实验、动物体内分布实验和病人ＲＩ研究证明，９９ｍＴｃ－Ｂ７２．３ＭＴ－１与９９ｍＴｃ－Ｂ７２．３两者所得结果基本一致，均可同样用于肺腺癌和胃腺癌的定位诊断。

おo evaluate the clinical diagnostic value of 99m Tc labeled bifunctional hybrid protein B723 MT1.The bifunctional hybrid protein B72.3 MT1 was produced by the fusion of mouse metallothionein1 cDNA gene into CH2 region of B72.3 heavy chain cDNA fragment using the recombinant DNA technology.The 99m Tc direct labeling method to B72.3 MT1 in cell binding assay in vitro,animal biodistribution and patient RⅡ were in accord with these of 99m TcB72.3 RⅡ of 99m TcB72.3 MT1 may be applied as a valuable method in the diagnosis of lung adenocarcinoma,gastroadenocarcinoma and may be useful for differentiating adenocarcinoma from nonadenocarcinoma.