摘要
目的研究杏仁对吸烟致DNA氧化损伤和脂质过氧化的保护作用。方法选取60名年龄在18～25岁男性吸烟志愿者,随机分为2组,每组30人,分别先后交叉给予安慰剂(100 g猪肉,相当于84 g杏仁所提供的热量)和84 g杏仁,均持续4周。分别通过HPLC法测定24 h尿样中8-羟基脱氧鸟苷(8-OHdG)和丙二醛(MDA)水平,进行彗星实验测定淋巴细胞DNA链的断裂情况。结果给吸烟者补充杏仁后,尿8-OHdG、MDA和淋巴细胞DNA的断裂分别降低了28%、23%和34%,血清维生素E升高了10%。结论杏仁能有效降低吸烟引起的DNA氧化损伤和脂质过氧化。
Objective To investigate the effect of almonds on oxidative DNA damage and lipid peroxidation induced by smoking. Methods Sixty male smokers aged 18-25 were recruited and divided into two groups （n = 30 ）. A randomized, placebo - controlled （ 100 g pork providing calories equal to the almond intake ） , cross - over clinical trial was conducted. Almonds （84 g/d） were added to the diet for 4 weeks. 8 -OHdG and MDA in 24 h urine were analyzed by HPLC, peripheral lymphocyte DNA strand breaks by comet assay. Results After almond consumption, urinary 8 - OHdG and MDA, peripheral lymphocyte DNA strand breaks were significandy decreased by 28% , 23% and 34% respectively. Serum vitamin E was significantly increased by 10%. Conclusion These results indicate that the consumption of almonds can diminish smoking - related oxidative DNA damage and lipid peroxidation.
出处
《中国预防医学杂志》
CAS
2009年第3期173-177,共5页
Chinese Preventive Medicine
基金
美国加州杏仁协会资助
