睡必妥对中枢神经系统的作用及吸入毒性观察

Effects of Shuibituo on central nervous system of mice and its inhalation toxicity

目的：观察睡必妥（Ｓｈｕｉｂｉｔｕｏ）吸入对小鼠中枢神经系统的作用及其吸入毒性和刺激性。方法：静式染毒柜小鼠吸入接触给药。ＹＳＤ－４Ｇ药理、生理实验多用仪测定实验小鼠自主活动，检测睡必妥与戊巴比妥钠的协同作用及对脑内５ＨＴ（５羟色胺），５ＨＩＡＡ（５羟吲哚乙酸）的影响。结果：睡必妥吸入能减少小鼠自主活动，与戊巴比妥钠具协同催眠作用，小鼠脑内５ＨＴ含量降低，５ＨＩＡＡ含量有增高趋势。睡必妥小鼠ＬＣ５０（２ｈ）大于７００００ｍｇ·ｍ－３，大鼠吸入接触睡必妥５９０００ｍｇ·ｍ－３４ｈ，连续３ｄ，对呼吸道粘膜组织无刺激性。结论：睡必妥具一定中枢抑制作用。其机制与苯二氮类安定药不同，可能与中枢神经递质５ＨＴ作用有关。睡必妥吸入毒性低。

OBJECTIVE: To study the effects of Shuibituo (a compound preparation of traditional Chinese medicine) on the central nervous system of mice and its inhalation toxicity. METHODS: Drugs were administered to the mice through the respiratory tract by a static intoxication equipment. Automatic activities (AA) of mice were recorded by a YSD-4G pharmacological physiological multi track recording equipment to evaluate the synergistic effects of Shuibituo and pentobarbital and the influence on the amount of cerebral 5 HT and 5 HIAA. RESULTS: Shuibituo reduced the AA of mice and exered a synergistic hypnotic effect with pentobarbital. There was a tendency in decreasing cerebral 5 HT and increasing 5 HIAA. LC 50 value for mice exceeded 70 000 mg·m -3 . No respiratory irritative action was observed in rats exposed to tranquil at 59 066 mg·m -3 , 4 h· -1 for 3 consecutive days. CONCLSION: Inhibitory effects on the CNS of mice were obseved. The mechanism was probably different from that of diazepam and may be related to the function of central neurotransmeter 5 hydroxy tryptamine. Shuibituo has low inhalation toxicity and no irritative action on the respiratory tract.