PDTC增强TRAIL对人Burkitt淋巴瘤细胞株Raji细胞凋亡的影响

The Augmentation Effect of PDTC on TRAIL-induced Apoptosis in Human Burkitt Lymphoma Cell Line Raji Cells

目的研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)联合吡咯烷二硫代氨基甲酸盐(PDTC)对EBV阳性的人Burkitt淋巴瘤细胞株Raji细胞凋亡的影响,并初步探讨其作用机制。方法应用MTT法检测TRAIL(1、10、100 ng/mL)及TRAIL(1、10、100 ng/mL)+100μmol/mL PDTC时Raji细胞的生长抑制率;原位末端酶标记技术(TUNEL)检测Raji凋亡细胞。结果①TRAIL 1、10 ng/mL组12 h Raji细胞生长抑制率分别为-35.52%及-15.07%;TRAIL 100 ng/mL组12 h Raji细胞生长抑制率为6.68%,并且呈时间依赖性(P<0.05)。②TRAIL(1、10、100 ng/mL)加入PDTC后,12 h Raji细胞生长抑制率分别为1.18%、4.96%、14.63%,均显著高于TRAIL单用组(均P<0.01)。③TRAIL(100 ng/mL)联合PDTC时Raji细胞凋亡指数最高为79.49%,较TRAIL100 ng/mL(28.84%)有显著性升高,1、10 ng/mL TRAIL联合PDTC后Raji凋亡细胞也均显著高于TRAIL单用组(均P<0.01)。与MTT法检测结果具有一致性。结论TRAIL能诱导Raji细胞凋亡但作用不敏感。PDTC能显著增强TRAIL对Raji细胞的诱凋亡作用。

Objective To investigate whether PDTC could increase the EBV positive human Burkitt lymphoma cell line Raji cells apoptosis induced by TRAIL and its underlying mechanism. Methods Raji cells were randomly divided into TRAIL groups and TRAIL-plus-PDTC groups. For TRAIL groups,cells were exposed to various concentrations of TRAIL （1,10,and 100 ng/mL respectively）. For TRAIL-plus-PDTC groups, were treated with 100 μmol/mL PDTC and TRAIL,the concentrations of which varied in accordance with that in TRAIL groups. MTT assay was then used to measure the cell growth inhibiting rate of Raji cells and terminal deoxynucleotide transferas-mediated aUTP nick end labeling （TUNEL） was used to examine the apoptosis index of the cells. Results （1）For TRAIL 1,10 ng/mL groups,the 12 h Raji cells growth inhibiting rate was respectively --35. 52% and --15.07%. In contrast, it was 6. 68% in TRAIL 100 ng/mL group. The cell growth inhibiting rate took on time-dependence（P〈0.05）. （2）For TRAIL-plus-PDTC groups, the 12 h Raji cells growth inhibiting rate was respectively 1.18 %, 4.96 %, 14.63%, the corresponding concentrations of TRAIL were 1, 10 and 100 ng/mL. Which was markedly higher than that in TRAIL groups（P〈0.01, respectively）. （3）The highest apoptosis index in Raji cells induced by 100 ng/mL TRAIL-plus-PDTC group was 79.49% which markedly higher than 28.84% in TRAIL 100 ng/mL group. The apoptosis index of Raji cells in 1 and 10 ng/mL TRAIL-plus-PDTC groups were also markedly higher than that in TRAIL groups （P〈0.01 ,respectively）. The results from both TUNEL and MTT were consistent. Conclusion Raji cells are insensitive to TRAIL in apoptosis, while PDTC could significantly strengthen this effect in Raji cells.