甘利欣注射液对哮喘大鼠支气管和肺组织形态及嗜酸粒细胞数的影响

Effect of Diammonium Glycyrrhizinate Injection on bronchus,lung tissue and amount of eosinophils(EOS)in rats with asthma

目的:观察甘利欣注射液对哮喘大鼠支气管和肺组织形态及嗜酸粒细胞(EOS)数的影响,探讨甘利欣注射液干预哮喘气道炎症的作用机制。方法:清洁级雄性SD大鼠60只,随机分为6组:正常对照组、模型对照组、地塞米松治疗组、大、中、小剂量甘利欣治疗组,每组10只,采用卵蛋白制作大鼠哮喘模型。各组给药2周后检测各组大鼠血清和支气管肺泡灌洗液中EOS数,观察支气管和肺组织的病理形态改变。结果:模型对照组EOS数明显高于正常对照组(P<0.05),支气管上皮损伤及基底膜增厚明显。与模型对照组比较,地塞米松治疗组与大、中剂量甘利欣治疗组均可不同程度的下调EOS数,支气管上皮损伤及基底膜增厚明显减轻。小剂量甘利欣治疗组则无此作用。结论:甘利欣注射液治疗哮喘气道炎症的作用机制可能与减少EOS对肺组织的浸润有关。

Objective： To observe the effect of diammonium glycyrrhizinate injection on bronchus, lung tissue and amount of eosinophils （EOS） in rats with asthma and to discuss the mechanism of diammonium glycyrrhizinate intervention on airway inflammation in asthma. Methods： To divide 60 male SD rats into 6 groups as following： normal control group, model group, dexameth group, high dose of diammonium glycyrrhizinate group, middle dose of diammonium glycyrrhizinate group and low dose of diammonium glycyrrhizinate group （n=10）. After setting up the model of rats with asthma, we detect the EOS in both rats＇ blood serum and bronchoalveolar lavage and observe the pathological change in bronchus and lung tissue in rats of each group. Results： Not only the amount of EOS of rats in model group is bigger than that in normal control group （P〈0.05） but the bronchio-epithelium injury and basal membrane thicken. Compared with the model group, dexameth, high dose and middle dose of diammonium glycyrrhizinate can decrease the EOS, relieve the Bronchio-epithelium injury and basal membranes thicken to some extent. Low dose of diammonium glycyrrhizinate do not have the above action. Conclusion： The mechanism of diammonium glycyrrhizinate intervention on airway inflammtion in asthma may be related with descrease of EOS infiltrating.