电针对老年性痴呆大鼠认知障碍的影响

Experimental study on effect of Ele-accupuncture on cognitive impairment of Alzheimer's disease model rats

目的:通过动物实验,观察电针"百会、风府、肾俞、悬钟"穴位对老年性痴呆大鼠行为学及血清丙二醛(MDA)及总抗氧化力(T-AOC)含量的影响,并进一步探讨其机制。方法:选用健康成年Wistar大鼠40只,将Wistar大鼠随机分为4组:模型组、电针组、西药组及针药结合组,腹腔注射D-半乳糖制备老年性痴呆模型大鼠。电针组:针刺百会、肾俞、风府、悬钟穴。西药组:脑复康灌胃,每日1次,隔日给予与电针组相同方式的固定和抓取。针药结合组:针刺治疗和药物治疗方法同上。模型组:每天给予电针组相同的抓取和固定。治疗后测试行为学及检测血清MDA、T-AOC的含量。结果:电针组、针药结合组能延长跳台实验潜伏期、减少错误次数(P<0.05),降低血清MDA及提高T-AOC含量(P<0.05)。结论:电针"百会、风府、肾俞、悬钟"可以延长跳台实验潜伏期、减少错误次数,降低血清MDA含量、提高T-AOC含量,其机制可能是通过增强氧自由基清除系统的功能,提高机体抗氧化能力实现的。

Objective： Through animal experiments to observe the behavior and serum malondialdehyde （MDA） and total antioxidative capacity （T-AOC） content after electro-acupuncture ＂Baihui, Fengfu, Shenshu, Xuanzhong＂ Point of Alzheimer＇s disease in rat. Method： Select forty Healthy adult Wistar rats, the Wistar rats were randomly divided into four groups,model group,electro-acupuncture group, western medicine group, electro-acupuncture and western medicine group. Abdominal cavity injection of D-galactose in order to prepare the Alzheimer＇s disease model rats. Electro-acupuncture group： acupuncture Baihui, Shenshu, Fengfu, Xuanzhong. Western medicine group： Piracetan intragastric administration one times a day.Every other day give the fixed and crawl for ten minutes. Electro-acupuncture and western medicine group： Accupuncture treatment and drug treatment are above all. Model group： Every day give the same fixed and crawl as electro-acupuncture group. Behavior and serum MDA, T-AOC levels tested after treatment. Results： electro-acupuncture group, electro-acupuncture and western medicine group could extend the incubation period, reduce the number of errors （P〈0.05）. lower the serum MDA and increase the T-AOC content （P〈0.05）. Conclusion： electro-acupuncture ＂Baihui, Fengfu, Shenshu, Xuanzhong＂ could extend the incubation period, reduce the number of errors, lower the serum MDA and increase the T-AOC content. The mechanism may be enhanced the function of oxygen free radical scavenging system, improve the ability of antioxidation.