大黄虫丸对小鼠Lewis肺癌CD44、VEGF及转移抑制的影响

Effects of Dahuang Zhechong Pills on CD44,VEGF and the metastasis suppressor in mice with Lewis Lung carcinoma

目的:通过观察大黄虫丸对小鼠Lewis肺癌细胞黏附因子(CD44)、血管内皮生长因子(VEGF)及转移抑制的影响,初步探讨其抗肿瘤转移的机制。方法:造模后次日先随机分成5组,每组10只。根据预试结果确定高(H)、中(M)、低(S)剂量分别为0.18、0.09、0.045g/ml,以每只0.2ml灌胃10d;空白组小鼠以NS0.2ml灌胃10d;化疗组以50mg/kg CTX于第1d予腹腔给药。第11d处死小鼠,肺玻片压片法观察肺表面的转移灶结节并计数。以免疫组化法检测VEGF的表达;以流式细胞仪测定CD44的活性,初步探讨其抗肿瘤的机制及作用靶点。结果:实验组高(H)、中(M)、低(L)剂量组和化疗组肺自发转移抑制率分别为57.44%、55.32%、38.72%、60.00%。CD44值分别为(0.59±0.86)、(0.21±0.28)、(0.33±0.31)、(1.88±1.36)实验组(M)低于空白组(M)及化疗组(P<0.05)。实验组(M)与空白组比,VEGF阳性表达率明显降低,差异有显著性(P<0.05)。结论:大黄虫丸降低C57BL小鼠Lewis肺癌肺转移发生率,实验组(M)作用明显。实验组(M)还具有抑制C57BL小鼠Lewis肺癌CD44活性,下调C57BL小鼠Lewis肺癌VEGF表达的作用。

Objective： By observing effects of Dahuang Zhechong Pills on CD44, VEGF and the metastasis suppressor in mice with Lewis Lung carcinoma, to discuss its mechanism of anti-tumor metastasis preliminarily. Methods： The mice were divided randomly into five groups the day after modeling and each group included ten mice. According to the pre-test, high-dose, medium-dose and low-dose were quantitated with 0.18g/ml, 0.09 g/ml, 0.045g/ml. The model group was intragastric administrated with NS 0.2ml for ten days; the chemotherapy group was Intraperitoneal administrated 50mg/kg CTX on the first day. Observing and numerating the pulmonary metastases with nodules by pulmonary pressure slide film on the 12th day. Based on irnmunohistochemical method to detect the expression of VEGF and the activity detection by flow cytometry CD44, Its antitumor mechanism and the role of target were preliminarily studied. Results： The inhibition rates of lung spontaneous metastasis in experimental groups of high （H）, medium （M）, low （L） dosages and the chemotherapy group were respectively 57.44 percent, 55.32 percent, 38.72 percent, 60.00 percent. CD44, respectively, values were 0.59 ±0.86, 0.21 ±0.28, 0.33 ± 0.31, 1.36± 0.52; the experimental group （M） compared with model group, VEGF expression was significantly reduced, there was a significant difference （P〈0.05）; VEGF expression rates of the experimental group （H）, the experimental group （L）, and chemotherapy group dropped, but the difference was not significant （P〉0.05）. Conclusion： Dahuang Zhechong Pills reduced the lung metastases incidence of C57BL mice with Lewis lung carcinoma and prolonged their lives. These results were obvious in the medium-dose experimental group. Mediumdose of Dahuang Zhechong Pills had effects on C57BL mice with Lewis lung carcinoma, whose CD44 activity were inhibited and the expression of VEGF was reduced.