摘要
目的分离制备人Ｃｏｔ┐１ＤＮＡ，用于肝癌相关基因的定位克隆研究。方法通过羟基磷灰石柱层析分离制备人Ｃｏｔ┐１ＤＮＡ。结果分离得到Ｃｏｔ值等于１的人基因组ＤＮＡ。结论采用羟基磷灰石柱层析法，可方便、快速、经济地分离得到人Ｃｏｔ┐１ＤＮＡ。
bjective:Human Cot1 DNA was prepared for positional cloning of human hepatomaassociated genes. Methods Human Cot1 DNA was isolated by column chromatography of hydroxyapatite. Results Human genomic DNA that Cot value is 1 was obtained. Conclusion Human Cot1 DNA was isolated conveniently,rapidly and economically by column chromatography of hydroxyapatite.
出处
《肿瘤》
CAS
CSCD
北大核心
1998年第3期127-128,共2页
Tumor
